Effect of Silver Nitrate Concentration on Production of Silver Nanoparticles Using Polygonum Minus Extract and Analysis of Their Antimicrobial Property
Green synthesis is the ability of organisms and organic compounds to reduce metal ions and stabilise them into nanoparticles (NPs). Among all metal nanoparticles, silver nanoparticles (AgNPs) have much attention due to the surface plasmon resonance (SPR), which can be easily observed by UV–visible spectrophotometer. In the present study, AgNPs were synthesized using Polygonum minus extract as a reducing agent and aqueous silver nitrate as a precursor. This study aims to investigate effect of silver nitrate (AgNO3) concentrations (0.001 M, 0.01 M and 0.1 M) on the production of AgNPs as well as an antimicrobial activity of silver nanoparticles (AgNPs). Based on the observation, the colorless reaction mixture slowly changed from yellowish green to reddish brown and further confirmed by surface plasmonic resonance (SPR) band at 420 - 440 nm using UV–visible spectroscopy indicating of reduction of silver ion after several minutes of reaction. It was found that increased concentration of AgNO3 resulted in increasing reaction time, production and decrease size of AgNPs. The morphological changes of bacterial cells treated with AgNPs were observed by FE-SEM and showed that the AgNPs has good antimicrobial properties against microorganisms. Thus, the ability of AgNPs to release Ag ions is a key to their antimicrobial.
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