SYNTHESIS OF SILVER NANOPARTICLES USING Polygonum minus EXTRACT AND ANALYSIS OF THEIR ANTIMICROBIAL PROPERTIES
Nanotechnology is a rapidly developing and promising field that makes best use of inert metals like silver, gold and platinum to synthesize metallic nanoparticles with high potential for various applications. Among all metal nanoparticles, silver nanoparticles (AgNPs) have much attention due to the surface plasmon resonance (SPR) (strong absorption in the visible region), which can be easily observed by UV–visible spectrophotometer. This study aims to investigate an antimicrobial activity of silver nanoparticles (AgNPs) synthesize using Polygonum minus extract as a reducing agent and aqueous silver nitrate as a precursor. Based on the observation, the colorless reaction mixture slowly changed from yellowish green to reddish brown and further confirmed by surface plasmonic resonance (SPR) band at 440 nm using UV–visible spectroscopy indicating of reduction of silver ion after several minutes of reaction. The AgNPs was characterized by Field-emission Scanning Electron Microscope (FE-SEM) and Transmission Electron Microscopy (TEM). The observation of FE-SEM showed the size of AgNPs was produced in the range of 15 nm – 25 nm while TEM image shows a well-dispersed silver nanoparticles with roughly spherical shape and size ranging particle size 6 – 21 nm. Three bacteria such as Staphylococcus aureus (ATCC 43300), Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 15442) were chosen to be tested in this study. The morphological changes of bacterial cells treated with AgNPs were observed by FE-SEM and showed that the AgNPs has good antimicrobial properties against microorganisms and it is proven by low of MIC value. Thus, the ability of AgNPs to release Ag ions is a key to their antimicrobial activity.
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