PRELIMINARY EXTRACTION OF CHLOROPHYLL A AND B FROM MICRO ALGAE ISOLATED FROM SELANGOR SEA WATER
Microalgae are organisms found in marine and freshwater environments. This organism is a natural source for a variable valuable compounds such as carbohydrates, proteins, lipids as well as pigments. Method for extraction of pigment from microalgae will be varied depending on the type of microalgae. The objective of this study was to obtain the chlorophyl a and b pigment from the two microalgae isolated from marine sample in Selangor. Several factors were tested to maximize the yield of pigments extraction, such as the solvent to be used, the cell wall disruption technique, the extraction time and the use of different empirical correlations. This study proposed to extract Chlorophyll a and b pigment from the sample by using methanol (99.5%), acetone (99.5%), and ethanol (95%) as the extraction solvents beside physical disruption using glass bead. Spectrophotometric analysis has been used to analyze and quantify the pigments. Chlorophyll a and chlorophyll b content of acetone extracted samples were higher than compared to ethanol and methanol. For sample 1, the results were given as 3.39 μg/mL and 2.80 μg/mL for chlorophyll a and chlorophyll b, while for sample 2, the results were given as 3.30 μg/mL and 2.95 μg/mL respectively.
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