Low-Density Polyethylene (LDPE) Degradation by Malaysian Rhodococcus spp. Using Weight Reduction Test
Keywords:
Low-density Polyethylene (LDPE) microbial degradation, Malaysian Rhodococcus spp., fermentation, plastic biodegradation
Abstract
As known, plastic accumulation worldwide is unbearable and becoming a huge environmental threat as they are very hard to be degraded. Low-Density Polyethylene (LDPE) is one of most common material used to produce plastic. This paper presents the investigation of the ability of twenty-three Malaysian Rhodococcus isolates in degrading low-density polyethylene (LDPE) through weight reduction test. Shake-flask incubation was performed for the investigation. Through the study, all isolates showed the ability to degrade the LDPE supplemented in the culture media but at different degradation rate. Among the 23 isolates, Rhodococcus UCC0018 demonstrated the highest degradation with 8.69 % of LDPE weight reduction. This finding shows the promising potential to explore this bacteria isolate as an agent for plastic waste biological treatment.
References
Abrusci C, Pablos J, Marín I, Espí E, Corrales T, Catalina F (2013) F, Comparative effect of metal stearates as pro-oxidant additives on bacterial biodegradation of thermal- and photodegraded low density polyethylene mulching films. Int Biodeterior Biodegrad 83:25–32. https ://doi.org/10.1016/j.ibiod.2013.04.002 Awasthi, S., Srivastava, N., Singh, T., Tiwary, D., & Mishra, P. (2017). Biodegradation of thermally treated low density polyethylene by fungus Rhizopus oryzae NS 5. 3 Biotech, 7(1). doi: 10.1007/s13205-017-0699-4 Carniel, A., Valoni, É., Junior, J. N., da Conceição Gomes, A., & de Castro, A. M. (2017). Lipase from Candida antarctica (CALB) and cutinase from Humicola insolens act synergistically for PET hydrolysis to terephthalic acid. Process Biochemistry, 59, 84-90. Da Luz JMR, Paes SA, Bazzolli DMS, Totola MR, Demuner AJ, Kasuya MCM (2014) Abiotic and biotic degradation of oxobiodegradable plastic bags by Pleurotus ostreatus. PLoS ONE. doi:10.1371/journal.pone.0107438 Danso, D., Chow, J., & Streit, W. R. (2019). Plastics: environmental and biotechnological perspectives on microbial degradation. Applied and Environmental Microbiology, 85(19). Das, M., & Kumar, S. (2014). Microbial Deterioration of Low Density Polyethylene by Aspergillus and Fusarium. International Journal of ChemTech Research. Emadian, S.M., Onat, T.T. and Demirel, B. (2017). Biodegradation of bioplastics in natural environments. Waste management. https://doi.org/10.1016/j.wasman.2016.10.006 Gajendiran, A., Krishnamoorthy, S., & Abraham, J. (2016). Microbial degradation of low-density polyethylene (LDPE) by Aspergillus clavatus strain JASK1 isolated from landfill soil. 3 Biotech, 6(1). doi: 10.1007/s13205-016-0394-x
Gu JD, Ford TE, Mitton DB, Mitchell R (2000) Microbial corrosion of metals. In: Review (ed) The Uhlig corrosion handbook, 2nd edn. Wiley, New York, pp 915–927 Das, M., & Kumar, S. (2014). An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens. 3 Biotech, 5(1), 81-86. doi: 10.1007/s13205-014-0205-1 Hadad, D., Geresh, S., & Sivan, A. (2005). Biodegradation of polyethylene by the thermophilic bacterium Brevibacillus borstelensis. Journal of Applied Microbiology, 98(5), 1093-1100. doi: 10.1111/j.1365-2672.2005.02553.xOrr, I., Hadar, Y., & Sivan, A. (2004). Colonization, biofilm formation and biodegradation of polyethylene by a strain of Rhodococcus ruber. Applied Microbiology And Biotechnology, 65(1). doi:10.1007/s00253-004-1584-8 Hasdianty, A., Suhaila, Y. N., Hazwan, A. H., Maniyam, M. N., Fadzli, A. M., & Ibrahim, A. L. (2020). Inferring the evolutionary relationship of 23 Malaysian Rhodococcus isolates with potential as cholesterol degrading bacteria. Biocatalysis and Agricultural Biotechnology, 101840. Harshvardhan, K., & Jha, B. (2013). Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India. Marine Pollution Bulletin, 77(1-2), 100-106.
Jasmin, A.F. and Wong, E.K. (2019). Plastic: Undegradable Problem. Accessed on 28th December 2020 at http://www.krinstitute.org/Views-@-Plastic-;_An_Undegradable_Problem.aspx
Jeon HJ, Kim MN (2014) Degradation of linear low density polyethylene (LLDPE) exposed to UV-irradiation. Eur Polym J 52:146–153. https ://doi.org/10.1016/j.eurpo lymj.2014.01.007 Jeon HJ, Kim MN (2015) Functional analysis of alkane hydroxylase system derived from Pseudomonas aeruginosa E7 for low molecular weight polyethylene biodegradation. Int Biodeterior Biodegrad 103:141–146. https ://doi.org/10.1016/j.ibiod.2015.04.024 Lee, Alicia, and Mei Shan Liew. "Tertiary recycling of plastics waste: an analysis of feedstock, chemical and biological degradation methods." Journal of Material Cycles and Waste Management (2020): 1-12. Montazer, Z., Habibi-Najafi, M. B., Mohebbi, M., & Oromiehei, A. (2018). Microbial degradation of UV-pretreated low-density polyethylene films by novel polyethylene-degrading bacteria isolated from plastic-dump soil. Journal of Polymers and the Environment, 26(9), 3613-3625.
Muenmee S, Chiemchaisri W, Chiemchaisri C (2015) Microbial consortium involving biological methane oxidation in relation to the biodegradation of waste plastics in a solid waste disposal open dump site. Int Biodeterior Biodegrad 102:172–181. https://doi.org/10.1016/j.ibiod .2015.03.015
Muenmee S, Chiemchaisri W, Chiemchaisri C (2016) Enhancement of biodegradation of plastic wastes via methane oxidation in semi-aerobic landfill. Int Biodeterior Biodegrad 113:244–255. https ://doi.org/10.1016/j.ibiod .2016.03.016 Nagarajan, J., Nawawi, N. M., & Ibrahim, A. L. Characterization of Lipase Producing Rhodococcus sp. from Peninsular Malaysia. Journal of Life Sciences and Technologies Vol. 2, No. 1. Nallapan Maniyam, M, Hazeeq Hazwan, A., Hasdianty, A. and Nor Suhaila, Y. (2020). Malaysian Rhodococcus as Promising Biological Tool for Wastewater Treatment. A book chapter in Industrial Wastewater: Industrial Pollutants, Treatment and Disposal. Nova Book Series. ISBN: 978-1-53618-252-1 Nanda, S. and Sahu, S.S. (2010). Biodegradibility of polyethylene by Brevibacillus, Pseudomonas, Rhodococcus spp. New York Science Journal, 3(7), 95-98
Sahebnazar Z, Shojaosadati SA, Mohammad-Taheri M, Nosrati M (2010) Biodegradation of low-density polyethylene (LDPE) by isolated fungi in solid waste medium. Waste Management 30:396–401. https ://doi.org/10.1016/j.wasma n.2009.09.027
Sen SK, Raut S (2015) Microbial degradation of low density polyethylene (LDPE): a review. J Environ Chem Eng 3:462–473. https ://doi.org/10.1016/j.jece.2015.01.003
Shah AA, Hasan F, Hameed A, Ahmed S (2008) Biological degradation of plastics: a comprehensive review. Biotechnol Adv 26:246–265. https ://doi.org/10.1016/j.biote chadv .2007.12.005
Sawadogo A, Harmonie OC, Sawadogo JB, Aminata K, Traoré AS, Dianou D (2014) Isolation and characterization of hydrocarbon-degrading bacteria from wastewaters in Ouagadougou, Burkina Faso. J Environ Prot 5:1183–1196. https ://doi. org/10.4236/jep.2014.51211 5 Sung CC, Tachibana Y, Suzuki M, Hsieh WC, Kasuya KI (2016). Identification of a poly(3 hydroxybutyrate)-degrading bacterium isolated from coastal seawater in Japan as Shewanella sp. Polym Degrad Stab 129:268–274. https ://doi.org/10.1016/j.polym degradstab .2016.05.008
Thirunavukarasu K, Purushothaman S, Sridevi J, Aarthy M, Gowthaman MK, Nakajima-Kambe T, Kamini NR (2016) Degradation of poly(butylene succinate) and poly(butylenes succinate-co-butylene adipate) by a lipase from yeast Cryptococcus sp. grown on agro-industrial residues. Int Biodeterior Biodegrad 110:99–107. https ://doi.org/10.1016/j.ibiod .2016.03.005
Tokiwa Y, Calabia BP, Ugwu CU, Aiba S (2009) Biodegradability of plastics. Int J Mol Sci 10:3722–3742. https ://doi.org/10.3390/ijms1 00937 22 Veethahavyaa KS, Rajath BS, Noobiab S, Kumar B (2016). Biodegradation of low density polyethylene in aqueous media. Proced Environ Sci 35:709–713. https ://doi.org/10.1016/j.proenv.2016.07.072
Vimala PP, Mathew L (2016) Biodegradation of polyethylene using Bacillus subtilis. Proced Technol 24:232–239. https ://doi.org/10.1016/j.protc y.2016.05.031
Watanabea T, Suzuki K, Shinozaki Y, Yarimizua T, Yoshidaa S, Sameshima-Yamashitaa Y, Koitabashi M, Kitamoto HK (2015) A UV-induced mutant of Cryptococcus flavus GB-1 with increased production of a biodegradable plastic-degrading enzyme. Process Biochem 50:1718–1724. https ://doi.org/10.1016/j.procbio.2015.07.005
Yang Y, Chen J, Wu WM, Zhao J, Yanga J (2016) Characterization of a thermolabile poly(3-hydroxybutyrate) depolymerase from the marine bacterium Shewanella sp. J KCM-AJ-6,1α. Polym Degrad Stab 129:212–221. https ://doi.org/10.1016/j.polym degradstab .2016.04.022
Gu JD, Ford TE, Mitton DB, Mitchell R (2000) Microbial corrosion of metals. In: Review (ed) The Uhlig corrosion handbook, 2nd edn. Wiley, New York, pp 915–927 Das, M., & Kumar, S. (2014). An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens. 3 Biotech, 5(1), 81-86. doi: 10.1007/s13205-014-0205-1 Hadad, D., Geresh, S., & Sivan, A. (2005). Biodegradation of polyethylene by the thermophilic bacterium Brevibacillus borstelensis. Journal of Applied Microbiology, 98(5), 1093-1100. doi: 10.1111/j.1365-2672.2005.02553.xOrr, I., Hadar, Y., & Sivan, A. (2004). Colonization, biofilm formation and biodegradation of polyethylene by a strain of Rhodococcus ruber. Applied Microbiology And Biotechnology, 65(1). doi:10.1007/s00253-004-1584-8 Hasdianty, A., Suhaila, Y. N., Hazwan, A. H., Maniyam, M. N., Fadzli, A. M., & Ibrahim, A. L. (2020). Inferring the evolutionary relationship of 23 Malaysian Rhodococcus isolates with potential as cholesterol degrading bacteria. Biocatalysis and Agricultural Biotechnology, 101840. Harshvardhan, K., & Jha, B. (2013). Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India. Marine Pollution Bulletin, 77(1-2), 100-106.
Jasmin, A.F. and Wong, E.K. (2019). Plastic: Undegradable Problem. Accessed on 28th December 2020 at http://www.krinstitute.org/Views-@-Plastic-;_An_Undegradable_Problem.aspx
Jeon HJ, Kim MN (2014) Degradation of linear low density polyethylene (LLDPE) exposed to UV-irradiation. Eur Polym J 52:146–153. https ://doi.org/10.1016/j.eurpo lymj.2014.01.007 Jeon HJ, Kim MN (2015) Functional analysis of alkane hydroxylase system derived from Pseudomonas aeruginosa E7 for low molecular weight polyethylene biodegradation. Int Biodeterior Biodegrad 103:141–146. https ://doi.org/10.1016/j.ibiod.2015.04.024 Lee, Alicia, and Mei Shan Liew. "Tertiary recycling of plastics waste: an analysis of feedstock, chemical and biological degradation methods." Journal of Material Cycles and Waste Management (2020): 1-12. Montazer, Z., Habibi-Najafi, M. B., Mohebbi, M., & Oromiehei, A. (2018). Microbial degradation of UV-pretreated low-density polyethylene films by novel polyethylene-degrading bacteria isolated from plastic-dump soil. Journal of Polymers and the Environment, 26(9), 3613-3625.
Muenmee S, Chiemchaisri W, Chiemchaisri C (2015) Microbial consortium involving biological methane oxidation in relation to the biodegradation of waste plastics in a solid waste disposal open dump site. Int Biodeterior Biodegrad 102:172–181. https://doi.org/10.1016/j.ibiod .2015.03.015
Muenmee S, Chiemchaisri W, Chiemchaisri C (2016) Enhancement of biodegradation of plastic wastes via methane oxidation in semi-aerobic landfill. Int Biodeterior Biodegrad 113:244–255. https ://doi.org/10.1016/j.ibiod .2016.03.016 Nagarajan, J., Nawawi, N. M., & Ibrahim, A. L. Characterization of Lipase Producing Rhodococcus sp. from Peninsular Malaysia. Journal of Life Sciences and Technologies Vol. 2, No. 1. Nallapan Maniyam, M, Hazeeq Hazwan, A., Hasdianty, A. and Nor Suhaila, Y. (2020). Malaysian Rhodococcus as Promising Biological Tool for Wastewater Treatment. A book chapter in Industrial Wastewater: Industrial Pollutants, Treatment and Disposal. Nova Book Series. ISBN: 978-1-53618-252-1 Nanda, S. and Sahu, S.S. (2010). Biodegradibility of polyethylene by Brevibacillus, Pseudomonas, Rhodococcus spp. New York Science Journal, 3(7), 95-98
Sahebnazar Z, Shojaosadati SA, Mohammad-Taheri M, Nosrati M (2010) Biodegradation of low-density polyethylene (LDPE) by isolated fungi in solid waste medium. Waste Management 30:396–401. https ://doi.org/10.1016/j.wasma n.2009.09.027
Sen SK, Raut S (2015) Microbial degradation of low density polyethylene (LDPE): a review. J Environ Chem Eng 3:462–473. https ://doi.org/10.1016/j.jece.2015.01.003
Shah AA, Hasan F, Hameed A, Ahmed S (2008) Biological degradation of plastics: a comprehensive review. Biotechnol Adv 26:246–265. https ://doi.org/10.1016/j.biote chadv .2007.12.005
Sawadogo A, Harmonie OC, Sawadogo JB, Aminata K, Traoré AS, Dianou D (2014) Isolation and characterization of hydrocarbon-degrading bacteria from wastewaters in Ouagadougou, Burkina Faso. J Environ Prot 5:1183–1196. https ://doi. org/10.4236/jep.2014.51211 5 Sung CC, Tachibana Y, Suzuki M, Hsieh WC, Kasuya KI (2016). Identification of a poly(3 hydroxybutyrate)-degrading bacterium isolated from coastal seawater in Japan as Shewanella sp. Polym Degrad Stab 129:268–274. https ://doi.org/10.1016/j.polym degradstab .2016.05.008
Thirunavukarasu K, Purushothaman S, Sridevi J, Aarthy M, Gowthaman MK, Nakajima-Kambe T, Kamini NR (2016) Degradation of poly(butylene succinate) and poly(butylenes succinate-co-butylene adipate) by a lipase from yeast Cryptococcus sp. grown on agro-industrial residues. Int Biodeterior Biodegrad 110:99–107. https ://doi.org/10.1016/j.ibiod .2016.03.005
Tokiwa Y, Calabia BP, Ugwu CU, Aiba S (2009) Biodegradability of plastics. Int J Mol Sci 10:3722–3742. https ://doi.org/10.3390/ijms1 00937 22 Veethahavyaa KS, Rajath BS, Noobiab S, Kumar B (2016). Biodegradation of low density polyethylene in aqueous media. Proced Environ Sci 35:709–713. https ://doi.org/10.1016/j.proenv.2016.07.072
Vimala PP, Mathew L (2016) Biodegradation of polyethylene using Bacillus subtilis. Proced Technol 24:232–239. https ://doi.org/10.1016/j.protc y.2016.05.031
Watanabea T, Suzuki K, Shinozaki Y, Yarimizua T, Yoshidaa S, Sameshima-Yamashitaa Y, Koitabashi M, Kitamoto HK (2015) A UV-induced mutant of Cryptococcus flavus GB-1 with increased production of a biodegradable plastic-degrading enzyme. Process Biochem 50:1718–1724. https ://doi.org/10.1016/j.procbio.2015.07.005
Yang Y, Chen J, Wu WM, Zhao J, Yanga J (2016) Characterization of a thermolabile poly(3-hydroxybutyrate) depolymerase from the marine bacterium Shewanella sp. J KCM-AJ-6,1α. Polym Degrad Stab 129:212–221. https ://doi.org/10.1016/j.polym degradstab .2016.04.022
Published
2021-04-20
How to Cite
Abdullah, H., Othman, N., Yaacob, N., Ahmad, M., Ibrahim, M., Maniyam, M., & Azman, H. (2021). Low-Density Polyethylene (LDPE) Degradation by Malaysian Rhodococcus spp. Using Weight Reduction Test. Selangor Science & Technology Review (SeSTeR), 5(3), 41-47. Retrieved from https://sester.journals.unisel.edu.my/ojs/index.php/sester/article/view/226
Section
Articles in English
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