Enhancement of C. vulgaris Species Growth Using Aquaculture Sludge Extracts
Abstract
High-value microalgae have many useful substances that can be used for many applications. This study established the effect of the sludge extract (SE) on C. vulgaris species. Five different autoclave extraction parameters were assessed on SEs, i.e. 1-h at 105 °C, 2-h at 105 °C, 1-h at 121 °C, 2-h at 121 °C, and 24-h at room temperature (natural extraction). The SE obtained from the Sabak Bernam (SB) and Kota Puteri (KP) was supplemented with Conway media and checked using microplate incubation technique. Microalgae cultivation in control (media) and enriched (media + SE) samples were incubated for nine days at 25 °C with 33.75 μmol photons m-2 s-2 light intensity on a 12-hour light: 12 h dark cycle. C. vulgaris (TRG 2A) and C. vulgaris (TRG6-B01) showed better growth in modified SE compared to control yet no significance differences (p > 0.05) were observed. The specific growth rate (SGR) of C. vulgaris (TRG 2A) showed significant differences (p < 0.05) between SB and KP SE while, C. vulgaris (TRG6-B01) showed no significant differences (p > 0.05). The organic matter contents in the SE and autoclave at extended high temperatures influences the microalgae growth.
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