Preliminary Extraction of Chlorophyll A and B from Micro Algae Isolated from Selangor Sea Water
Abstract
Microalgae are organisms found in marine and freshwater environments. This organism is a natural source for a variable valuable compounds such as carbohydrates, proteins, lipids as well as pigments. Method for extraction of pigment from microalgae will be varied depending on the type of microalgae. The objective of this study was to obtain the chlorophyl a and b pigment from the two microalgae isolated from marine sample in Selangor. Several factors were tested to maximize the yield of pigments extraction, such as the solvent to be used, the cell wall disruption technique, the extraction time and the use of different empirical correlations. This study proposed to extract Chlorophyll a and b pigment from the sample by using methanol (99.5%), acetone (99.5%), and ethanol (95%) as the extraction solvents beside physical disruption using glass bead. Spectrophotometric analysis has been used to analyze and quantify the pigments. Chlorophyll a and chlorophyll b content of acetone extracted samples were higher than compared to ethanol and methanol. For sample 1, the results were given as 3.39 μg/mL and 2.80 μg/mL for chlorophyll a and chlorophyll b, while for sample 2, the results were given as 3.30 μg/mL and 2.95 μg/mL respectively.
References
Chan, J. 2012. Extracting lipid and carotenoids from microalgae with lecithin-linker microemulsions (Master thesis)Canada: Department of Chemical Engineering and Applied Chemistry, University of Toronto.
Sartory D. P. and Grobbelaar J. U. 1984. Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis,” Hydrobiologia, vol. 114, no. 3, pp. 177–187.
Ghosh, P., Das, P., Mukherjee, R., Banik, S., Karmakar, S. and Chatterjee, S. 2018. Extraction and Quantification of Pigments From Indian Traditional. International Journal of Pharmaceutical Sciences and Research 9(7): 3052–3059.
Halim, R., Hosikian, A., Lim, S. and Danquah, M. K. 2010. Chlorophyll extraction from microalgae: A review on the process engineering aspects. International Journal of Chemical Engineering 2209–2222.
Henriques, M., Silva, a and Rocha, J. 2007a. Extraction and quantification of pigments from a marine microalga : a simple and reproducible method. Communicating Current Research and Educational Topics and Trends in Applied Microbiology 586–593.
Ilavarasi, A., Mubarakali, D., Praveenkumar, R., Baldev, E. and Thajuddin, N. 2011. Optimization of various growth media to freshwater microalgae for biomass production. Biotechnology 10(6): 540–545.
Jeffrey S. W., Mantoura R. F. C. and Wright S. W. 1997. Eds., Phy toplankton Pigments in Oceanography: Guidelines to Modern Methods, UNESCO, Paris, France.
Lichtenthaler, H. K. 1987. Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes. Methods in Enzymology 148(C): 350–382.
Marilum, M. C. and Products, M. S. 2019. Essential Science : Why algae may be the
superfood of 2017: 1–2.
Maurya, S. S., Maurya, J. N., & Pandey, V. D. (2014). Factors regulating phycobiliprotein production in cyanobacteria. International Journal of Current Microbiology and Applied Sciences, 3(5), 764–771.
Simon D.and Helliwell S. 1998. Extraction and quantification of chlorophyll a from freshwater green algae. Water Research, vol. 32, no. 7, pp. 2220–2223.
Singh, B., Liu, Y. and Sharma, Y. C. 2013. Synthesis of biodiesel/bio-oil from microalgae. Biotechnological Applications of Microalgae: Biodiesel and Value-Added Products 6(126): 99–112. https://doi.org/10.1201/b14
All materials contained within this journal are protected by Intellectual Property Corporation of Malaysia, Copyright Act 1987 and may not be reproduced, distributed, transmitted, displayed, published, or
broadcast without the prior, express written permission of Centre for Graduate Studies, Universiti Selangor, Malaysia. You may not alter or remove any copyright or other notice from copies of this content.
