Effect on Growth of Desmodesmus sp Under Different Incubation Conditions

Wan Muhammad Ikram; Fridelina Sjahrir; Noor Fazreen Dzulkafli; Shuhaila Yaacob; Kazuhiro Komatsu; Emi Fazlina Hashim

Wan Muhammad Ikram
Fridelina Sjahrir
Noor Fazreen Dzulkafli
Shuhaila Yaacob
Kazuhiro Komatsu
Emi Fazlina Hashim

Keywords: Desmodesmus sp., Light Intensity, Optical Density, pH, Specific Growth Rate, Temperature

Abstract

The aim of the research was to find the best conditions for selected microalgae species, Desmodesmus. sp in term of growth productivity in different pH, temperature and intensity of light. Desmodesmus. Sp (TRG-D01) was isolated from Setiu Wetlands, Terengganu and cultured using Bold Basal Media (BBM). The species was incubated under different selected parameters for nine days using microplate reader technique through Optical Density (OD) measurement. From the experiment, Desmodesmus. sp showed variety of growth pattern. Incubation under different pH, light intensity and temperature showed specific growth rate ranging from 0.21 to 0.47 d-1 (maximum OD 0.67 ̶ 0.93), 0.33 to 0.43 d-1 (maximum OD 1.12) and 0.12 to 0.42 d-1 (maximum OD=1.18) respectively. Desmodesmus. sp was optimum in were observed when incubated under pH in between 7 to 9, 56 to 91

References

Bakuei, N., Amini, G., Najafpour, G. D., Jahanshahi, M., & Mohammadi, M. (2015). Optimal cultivation of Scenedesmus sp. microalgae in a bubble column photobioreactor.
Bohutskyi, P., Liu, K., Nasr, L. K., Byers, N., Rosenberg, J. N., Oyler, G. A., ... & Bouwer, E. J. (2015). Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate. Applied microbiology and biotechnology, 99(14), 6139-6154.
Demirel, Z., Yilmaz, F. F., Ozdemir, G., & Dalay, M. C. (2018). Influence of Media and Temperature on the Growth and the Biological Activities of Desmodesmus protuberans (FE Fritsch & MF Rich) E. Hegewald. Turkish Journal of Fisheries and Aquatic Sciences, 18(10), 1195-1203.
Difusa, A., Talukdar, J., Kalita, M. C., Mohanty, K., & Goud, V. V. (2015). Effect of light intensity and pH condition on the growth, biomass and lipid content of microalgae Scenedesmus species. Biofuels, 6(1-2), 37-44.
Ding, G. T., Takriff, M. S., Salihon, S., Syukri, M., & Rahaman, A. B. D. (2015). Feasiblity of the optical density (OD) in the determination of the microalgal biomass using palm oil mill effluent (POME) as medium. In Proceedings of 50th the IIER International Conference, Zurich, Switzerland, 26th December.
El-Sheekh, M., Abomohra, A. E. F., Abd El-Azim, M., & Abou-Shanab, R. (2017). Effect of temperature on growth and fatty acids profile of the biodiesel producing microalga Scenedesmus acutus. BASE.
He, Q., Yang, H., Wu, L., & Hu, C. (2015). Effect of light intensity on physiological changes, carbon allocation and neutral lipid accumulation in oleaginous microalgae. Bioresource technology, 191, 219-228.
Komatsu, Kazuhiro., Onouchi, Hidemi., Imai, Akio., Kawasaki, Nobuyuki., Hashim, Emi & Rajuddin, Mohd. (2019). Effects of Dissolved Organic Matter in Soil Extracts on the Growth of Microalgae土壌抽出物中溶存有機物(DOM) の微細藻類増殖に及ぼす影響. Journal of Japan Society on Water Environment. 42. 239-246. 10.2965/jswe.42.239.
Maynardo, J. J., Doshi, V. E. E. N. A., Rajanren, J. R., & Rajasekaran, R. (2015). The Optimization of Light Intensity and Drying Temperature on Lipid Content of Microalgae Nannochloropsis oculata. J Eng Sci Technol, 112-121.
Munoz, R., & Guieysse, B. (2006). Algal–bacterial processes for the treatment of hazardous contaminants: a review. Water research, 40(15), 2799-2815.
Ren, T. (2014). Primary factors affecting growth of microalgae optimal light exposure duration and frequency. Master thesis, Iowa State University.
Rios, L. F., Klein, B. C., Luz, L. F., Maciel Filho, R., & Maciel, M. W. (2015). Nitrogen starvation for lipid accumulation in the microalga species Desmodesmus spp. Applied biochemistry and biotechnology, 175(1), 469-476. https://dx.doi.org/10.1007/s12010-014- 1283-6
Sharma, A. K., Sahoo, P. K., Singhal, S., & Patel, A. (2016). Impact of various media and organic carbon sources on biofuel production potential from Chlorella spp. 3 Biotech, 6(2), 116.
Van Wagenen, J., Holdt, S. L., De Francisci, D., Valverde-Pérez, B., Plósz, B. G., & Angelidaki, I. (2014). Microplate-based method for high-throughput screening of microalgae growth potential. Bioresource technology, 169, 566-572.
Vanags, J., Kunga, L., Dubencovs, K., Galvanauskas, V., & Grīgs, O. (2015). Influence of light intensity and temperature on cultivation of microalgae Desmodesmus Communis in flasks and laboratory-scale stirred tank photobioreactor. Latvian Journal of Physics and Technical Sciences, 52(2), 59-70.
Wahidin, S., Idris, A., & Shaleh, S. R. M. (2013). The influence of light intensity and photoperiod on the growth and lipid content of microalgae Nannochloropsis sp. Bioresource technology, 129, 7-11.