Genetic Variation among F1 and F2 Parents Stocks from Cultured Populations of Lates calcarifer in Tok Bali, Kelantan using DNA Polymorphism Markers

Abstract

eabass belongs to the species of Lates calcarifer which is one of the economically marine food fish species in tropical and subtropical regions of Asia Pacific. It has been produced widely in large scale for domestic and export market. Malaysia is one of the major producers of cultured seabass in Asia.  In this study, DNA polymorphism markers are used to determine the genetic divergences of the species among the F1 and F2 parents stocks in DIMAN SDN BHD at Tok Bali, Kelantan. After screening, seven primers (OPA 02, OPA 03, OPA 05, OPA 09, OPA 10, OPA13, and OPA 18) were selected and used to analyze the polymorphisms of seabass. The RAPD analyses showed that the genetic distance among three cultured populations (F1/1, F2/1 and F2/2) of L. calcarifer was 0.202-0.252 indicating that all populations in the hatchery are genetically closely related. The value of genetic distance among individuals of L. calcarifer showed high genetic distance between F1/1(3), male individual and F2/2(1), female individual (0.429). The result obtained through this study can serve as a reference to DIMAN SDN BHD when applying selective breeding program and act as a supportive research in reducing inbreeding depression.